steponeplus™ realtime pcr system Search Results


steponeplus realtime pcr system  (Thermo Fisher)
99
Thermo Fisher steponeplus realtime pcr system
Steponeplus Realtime Pcr System, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/steponeplus%E2%84%A2+realtime+pcr+system/Phosphate/pm26663020-53-6-40
Average 99 stars, based on 1 article reviews
steponeplus realtime pcr system - by Bioz Stars, 2026-09
99/100 stars
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realtime pcr  (Sangon Biotech)
86
Sangon Biotech realtime pcr
Realtime Pcr, supplied by Sangon Biotech, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/steponeplus%E2%84%A2+realtime+pcr+system/pcr+realtime/pm36049215-79-5-34
Average 86 stars, based on 1 article reviews
realtime pcr - by Bioz Stars, 2026-09
86/100 stars
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realtime pcr  (SCAPS GmbH)
90
SCAPS GmbH realtime pcr
Effect <t>of</t> <t>BMP-4</t> on the viability of SCAPs. (A) not confluent SCAPs (1 × 10 4 cells/well) were exposed to BMP-4 for 5 days. (B) Near confluent SCAPs (1 × 10 5 cells/well) were exposed to BMP-4 for 5 days. Cell viability was estimated by MTT assay. Results were expressed as % of control (as 100 %). ∗denotes statistically significant difference when compared with solvent control group. (C) Morphology of SCAPs in cultured medium for 5 days, (D) Morphology of SCAPs after exposure to BMP-4 (200 ng/ml) for 5 days. One representative picture was shown. Effect of BMP-4 on the mRNA expression of various differentiation markers as analyzed by realtime <t>PCR.</t> (E) Osterix expression, (F) N-cadherin expression, (G) CTGF expression, (H) SPARC expression in SCAPs. Results were expressed as fold of control (as 1). ∗denotes statistically significant difference when compared with control. BMP-4: bone morphogenetic protein-4; SCAP: stem cells from apical papilla; MTT: 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide; CTGF: connective tissue growth factor; SPARC: secreted protein acidic and rich in cysteine.
Realtime Pcr, supplied by SCAPS GmbH, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/steponeplus%E2%84%A2+realtime+pcr+system/real+time+rt+pcr/pmc11762932-106-14-29
Average 90 stars, based on 1 article reviews
realtime pcr - by Bioz Stars, 2026-09
90/100 stars
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quantitative realtime pcr alleleseqr suite  (Celera)
90
Celera quantitative realtime pcr alleleseqr suite
Effect <t>of</t> <t>BMP-4</t> on the viability of SCAPs. (A) not confluent SCAPs (1 × 10 4 cells/well) were exposed to BMP-4 for 5 days. (B) Near confluent SCAPs (1 × 10 5 cells/well) were exposed to BMP-4 for 5 days. Cell viability was estimated by MTT assay. Results were expressed as % of control (as 100 %). ∗denotes statistically significant difference when compared with solvent control group. (C) Morphology of SCAPs in cultured medium for 5 days, (D) Morphology of SCAPs after exposure to BMP-4 (200 ng/ml) for 5 days. One representative picture was shown. Effect of BMP-4 on the mRNA expression of various differentiation markers as analyzed by realtime <t>PCR.</t> (E) Osterix expression, (F) N-cadherin expression, (G) CTGF expression, (H) SPARC expression in SCAPs. Results were expressed as fold of control (as 1). ∗denotes statistically significant difference when compared with control. BMP-4: bone morphogenetic protein-4; SCAP: stem cells from apical papilla; MTT: 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide; CTGF: connective tissue growth factor; SPARC: secreted protein acidic and rich in cysteine.
Quantitative Realtime Pcr Alleleseqr Suite, supplied by Celera, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/steponeplus%E2%84%A2+realtime+pcr+system/quantitative+realtime+pcr+alleleseqr+suite/10__1111_slash_j__1399___0039__2012__01877__x-1122-8-12
Average 90 stars, based on 1 article reviews
quantitative realtime pcr alleleseqr suite - by Bioz Stars, 2026-09
90/100 stars
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realtime pcr  (National Reference Center for Legionella)
90
National Reference Center for Legionella realtime pcr
Effect <t>of</t> <t>BMP-4</t> on the viability of SCAPs. (A) not confluent SCAPs (1 × 10 4 cells/well) were exposed to BMP-4 for 5 days. (B) Near confluent SCAPs (1 × 10 5 cells/well) were exposed to BMP-4 for 5 days. Cell viability was estimated by MTT assay. Results were expressed as % of control (as 100 %). ∗denotes statistically significant difference when compared with solvent control group. (C) Morphology of SCAPs in cultured medium for 5 days, (D) Morphology of SCAPs after exposure to BMP-4 (200 ng/ml) for 5 days. One representative picture was shown. Effect of BMP-4 on the mRNA expression of various differentiation markers as analyzed by realtime <t>PCR.</t> (E) Osterix expression, (F) N-cadherin expression, (G) CTGF expression, (H) SPARC expression in SCAPs. Results were expressed as fold of control (as 1). ∗denotes statistically significant difference when compared with control. BMP-4: bone morphogenetic protein-4; SCAP: stem cells from apical papilla; MTT: 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide; CTGF: connective tissue growth factor; SPARC: secreted protein acidic and rich in cysteine.
Realtime Pcr, supplied by National Reference Center for Legionella, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/steponeplus%E2%84%A2+realtime+pcr+system/real+time+pcr/10__1111_slash_j__1574___695x__2011__00899__x-14-11-16
Average 90 stars, based on 1 article reviews
realtime pcr - by Bioz Stars, 2026-09
90/100 stars
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realtime polymerase chain reaction (pcr) assay  (Proflu Inc)
90
Proflu Inc realtime polymerase chain reaction (pcr) assay
Effect <t>of</t> <t>BMP-4</t> on the viability of SCAPs. (A) not confluent SCAPs (1 × 10 4 cells/well) were exposed to BMP-4 for 5 days. (B) Near confluent SCAPs (1 × 10 5 cells/well) were exposed to BMP-4 for 5 days. Cell viability was estimated by MTT assay. Results were expressed as % of control (as 100 %). ∗denotes statistically significant difference when compared with solvent control group. (C) Morphology of SCAPs in cultured medium for 5 days, (D) Morphology of SCAPs after exposure to BMP-4 (200 ng/ml) for 5 days. One representative picture was shown. Effect of BMP-4 on the mRNA expression of various differentiation markers as analyzed by realtime <t>PCR.</t> (E) Osterix expression, (F) N-cadherin expression, (G) CTGF expression, (H) SPARC expression in SCAPs. Results were expressed as fold of control (as 1). ∗denotes statistically significant difference when compared with control. BMP-4: bone morphogenetic protein-4; SCAP: stem cells from apical papilla; MTT: 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide; CTGF: connective tissue growth factor; SPARC: secreted protein acidic and rich in cysteine.
Realtime Polymerase Chain Reaction (Pcr) Assay, supplied by Proflu Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/steponeplus%E2%84%A2+realtime+pcr+system/realtime+polymerase+chain+reaction++pcr++assay/pm22998763-21-8-14
Average 90 stars, based on 1 article reviews
realtime polymerase chain reaction (pcr) assay - by Bioz Stars, 2026-09
90/100 stars
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chi-square  (CH Instruments)
90
CH Instruments chi-square
Effect <t>of</t> <t>BMP-4</t> on the viability of SCAPs. (A) not confluent SCAPs (1 × 10 4 cells/well) were exposed to BMP-4 for 5 days. (B) Near confluent SCAPs (1 × 10 5 cells/well) were exposed to BMP-4 for 5 days. Cell viability was estimated by MTT assay. Results were expressed as % of control (as 100 %). ∗denotes statistically significant difference when compared with solvent control group. (C) Morphology of SCAPs in cultured medium for 5 days, (D) Morphology of SCAPs after exposure to BMP-4 (200 ng/ml) for 5 days. One representative picture was shown. Effect of BMP-4 on the mRNA expression of various differentiation markers as analyzed by realtime <t>PCR.</t> (E) Osterix expression, (F) N-cadherin expression, (G) CTGF expression, (H) SPARC expression in SCAPs. Results were expressed as fold of control (as 1). ∗denotes statistically significant difference when compared with control. BMP-4: bone morphogenetic protein-4; SCAP: stem cells from apical papilla; MTT: 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide; CTGF: connective tissue growth factor; SPARC: secreted protein acidic and rich in cysteine.
Chi Square, supplied by CH Instruments, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/steponeplus%E2%84%A2+realtime+pcr+system/chi+square/pm31301290-47-57-30
Average 90 stars, based on 1 article reviews
chi-square - by Bioz Stars, 2026-09
90/100 stars
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realtime pcr  (Klingspor Abrasives)
90
Klingspor Abrasives realtime pcr
Effect <t>of</t> <t>BMP-4</t> on the viability of SCAPs. (A) not confluent SCAPs (1 × 10 4 cells/well) were exposed to BMP-4 for 5 days. (B) Near confluent SCAPs (1 × 10 5 cells/well) were exposed to BMP-4 for 5 days. Cell viability was estimated by MTT assay. Results were expressed as % of control (as 100 %). ∗denotes statistically significant difference when compared with solvent control group. (C) Morphology of SCAPs in cultured medium for 5 days, (D) Morphology of SCAPs after exposure to BMP-4 (200 ng/ml) for 5 days. One representative picture was shown. Effect of BMP-4 on the mRNA expression of various differentiation markers as analyzed by realtime <t>PCR.</t> (E) Osterix expression, (F) N-cadherin expression, (G) CTGF expression, (H) SPARC expression in SCAPs. Results were expressed as fold of control (as 1). ∗denotes statistically significant difference when compared with control. BMP-4: bone morphogenetic protein-4; SCAP: stem cells from apical papilla; MTT: 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide; CTGF: connective tissue growth factor; SPARC: secreted protein acidic and rich in cysteine.
Realtime Pcr, supplied by Klingspor Abrasives, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/steponeplus%E2%84%A2+realtime+pcr+system/realtime+pcr/pm18287291-11-3-18
Average 90 stars, based on 1 article reviews
realtime pcr - by Bioz Stars, 2026-09
90/100 stars
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molgen sars-cov-2 (3genes) realtime pcr  (Adaltis Inc)
90
Adaltis Inc molgen sars-cov-2 (3genes) realtime pcr
Effect <t>of</t> <t>BMP-4</t> on the viability of SCAPs. (A) not confluent SCAPs (1 × 10 4 cells/well) were exposed to BMP-4 for 5 days. (B) Near confluent SCAPs (1 × 10 5 cells/well) were exposed to BMP-4 for 5 days. Cell viability was estimated by MTT assay. Results were expressed as % of control (as 100 %). ∗denotes statistically significant difference when compared with solvent control group. (C) Morphology of SCAPs in cultured medium for 5 days, (D) Morphology of SCAPs after exposure to BMP-4 (200 ng/ml) for 5 days. One representative picture was shown. Effect of BMP-4 on the mRNA expression of various differentiation markers as analyzed by realtime <t>PCR.</t> (E) Osterix expression, (F) N-cadherin expression, (G) CTGF expression, (H) SPARC expression in SCAPs. Results were expressed as fold of control (as 1). ∗denotes statistically significant difference when compared with control. BMP-4: bone morphogenetic protein-4; SCAP: stem cells from apical papilla; MTT: 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide; CTGF: connective tissue growth factor; SPARC: secreted protein acidic and rich in cysteine.
Molgen Sars Cov 2 (3genes) Realtime Pcr, supplied by Adaltis Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/steponeplus%E2%84%A2+realtime+pcr+system/molgen+sars+cov+2++3genes++realtime+pcr/pmc08884839-4-7-3
Average 90 stars, based on 1 article reviews
molgen sars-cov-2 (3genes) realtime pcr - by Bioz Stars, 2026-09
90/100 stars
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quantitative realtime pcr  (Ribobio co)
90
Ribobio co quantitative realtime pcr
Effect <t>of</t> <t>BMP-4</t> on the viability of SCAPs. (A) not confluent SCAPs (1 × 10 4 cells/well) were exposed to BMP-4 for 5 days. (B) Near confluent SCAPs (1 × 10 5 cells/well) were exposed to BMP-4 for 5 days. Cell viability was estimated by MTT assay. Results were expressed as % of control (as 100 %). ∗denotes statistically significant difference when compared with solvent control group. (C) Morphology of SCAPs in cultured medium for 5 days, (D) Morphology of SCAPs after exposure to BMP-4 (200 ng/ml) for 5 days. One representative picture was shown. Effect of BMP-4 on the mRNA expression of various differentiation markers as analyzed by realtime <t>PCR.</t> (E) Osterix expression, (F) N-cadherin expression, (G) CTGF expression, (H) SPARC expression in SCAPs. Results were expressed as fold of control (as 1). ∗denotes statistically significant difference when compared with control. BMP-4: bone morphogenetic protein-4; SCAP: stem cells from apical papilla; MTT: 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide; CTGF: connective tissue growth factor; SPARC: secreted protein acidic and rich in cysteine.
Quantitative Realtime Pcr, supplied by Ribobio co, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/steponeplus%E2%84%A2+realtime+pcr+system/quantitative+real+time+pcr/pm22761738-230-7-14
Average 90 stars, based on 1 article reviews
quantitative realtime pcr - by Bioz Stars, 2026-09
90/100 stars
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the primers were all designed by idt (http://sg.idtdna.com/site) online “realtime pcr tool” and purchased from bgi (shenzhen).  (BGI Shenzhen)
90
BGI Shenzhen the primers were all designed by idt (http://sg.idtdna.com/site) online “realtime pcr tool” and purchased from bgi (shenzhen).
Effect <t>of</t> <t>BMP-4</t> on the viability of SCAPs. (A) not confluent SCAPs (1 × 10 4 cells/well) were exposed to BMP-4 for 5 days. (B) Near confluent SCAPs (1 × 10 5 cells/well) were exposed to BMP-4 for 5 days. Cell viability was estimated by MTT assay. Results were expressed as % of control (as 100 %). ∗denotes statistically significant difference when compared with solvent control group. (C) Morphology of SCAPs in cultured medium for 5 days, (D) Morphology of SCAPs after exposure to BMP-4 (200 ng/ml) for 5 days. One representative picture was shown. Effect of BMP-4 on the mRNA expression of various differentiation markers as analyzed by realtime <t>PCR.</t> (E) Osterix expression, (F) N-cadherin expression, (G) CTGF expression, (H) SPARC expression in SCAPs. Results were expressed as fold of control (as 1). ∗denotes statistically significant difference when compared with control. BMP-4: bone morphogenetic protein-4; SCAP: stem cells from apical papilla; MTT: 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide; CTGF: connective tissue growth factor; SPARC: secreted protein acidic and rich in cysteine.
The Primers Were All Designed By Idt (Http://Sg.Idtdna.Com/Site) Online “Realtime Pcr Tool” And Purchased From Bgi (Shenzhen)., supplied by BGI Shenzhen, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/steponeplus%E2%84%A2+realtime+pcr+system/the+primers+were+all+designed+by+idt++http+++sg+idtdna+com+site++online+%E2%80%9Crealtime+pcr+tool++and+purchased+from+bgi++shenzhen++/pm32767810-75-9-15
Average 90 stars, based on 1 article reviews
the primers were all designed by idt (http://sg.idtdna.com/site) online “realtime pcr tool” and purchased from bgi (shenzhen). - by Bioz Stars, 2026-09
90/100 stars
  Buy from Supplier
realtime pcr primers  (TIB MOLBIOL)
90
TIB MOLBIOL realtime pcr primers
Effect <t>of</t> <t>BMP-4</t> on the viability of SCAPs. (A) not confluent SCAPs (1 × 10 4 cells/well) were exposed to BMP-4 for 5 days. (B) Near confluent SCAPs (1 × 10 5 cells/well) were exposed to BMP-4 for 5 days. Cell viability was estimated by MTT assay. Results were expressed as % of control (as 100 %). ∗denotes statistically significant difference when compared with solvent control group. (C) Morphology of SCAPs in cultured medium for 5 days, (D) Morphology of SCAPs after exposure to BMP-4 (200 ng/ml) for 5 days. One representative picture was shown. Effect of BMP-4 on the mRNA expression of various differentiation markers as analyzed by realtime <t>PCR.</t> (E) Osterix expression, (F) N-cadherin expression, (G) CTGF expression, (H) SPARC expression in SCAPs. Results were expressed as fold of control (as 1). ∗denotes statistically significant difference when compared with control. BMP-4: bone morphogenetic protein-4; SCAP: stem cells from apical papilla; MTT: 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide; CTGF: connective tissue growth factor; SPARC: secreted protein acidic and rich in cysteine.
Realtime Pcr Primers, supplied by TIB MOLBIOL, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/steponeplus%E2%84%A2+realtime+pcr+system/rt+pcr+primers/pmc04203231-60-0-6
Average 90 stars, based on 1 article reviews
realtime pcr primers - by Bioz Stars, 2026-09
90/100 stars
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Image Search Results


Effect of BMP-4 on the viability of SCAPs. (A) not confluent SCAPs (1 × 10 4 cells/well) were exposed to BMP-4 for 5 days. (B) Near confluent SCAPs (1 × 10 5 cells/well) were exposed to BMP-4 for 5 days. Cell viability was estimated by MTT assay. Results were expressed as % of control (as 100 %). ∗denotes statistically significant difference when compared with solvent control group. (C) Morphology of SCAPs in cultured medium for 5 days, (D) Morphology of SCAPs after exposure to BMP-4 (200 ng/ml) for 5 days. One representative picture was shown. Effect of BMP-4 on the mRNA expression of various differentiation markers as analyzed by realtime PCR. (E) Osterix expression, (F) N-cadherin expression, (G) CTGF expression, (H) SPARC expression in SCAPs. Results were expressed as fold of control (as 1). ∗denotes statistically significant difference when compared with control. BMP-4: bone morphogenetic protein-4; SCAP: stem cells from apical papilla; MTT: 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide; CTGF: connective tissue growth factor; SPARC: secreted protein acidic and rich in cysteine.

Journal: Journal of Dental Sciences

Article Title: Bone morphogenetic protein-4 induced matrix turnover and osteogenic differentiation-related molecules of stem cells from apical papilla and its associated ALK/Smad signaling

doi: 10.1016/j.jds.2024.11.002

Figure Lengend Snippet: Effect of BMP-4 on the viability of SCAPs. (A) not confluent SCAPs (1 × 10 4 cells/well) were exposed to BMP-4 for 5 days. (B) Near confluent SCAPs (1 × 10 5 cells/well) were exposed to BMP-4 for 5 days. Cell viability was estimated by MTT assay. Results were expressed as % of control (as 100 %). ∗denotes statistically significant difference when compared with solvent control group. (C) Morphology of SCAPs in cultured medium for 5 days, (D) Morphology of SCAPs after exposure to BMP-4 (200 ng/ml) for 5 days. One representative picture was shown. Effect of BMP-4 on the mRNA expression of various differentiation markers as analyzed by realtime PCR. (E) Osterix expression, (F) N-cadherin expression, (G) CTGF expression, (H) SPARC expression in SCAPs. Results were expressed as fold of control (as 1). ∗denotes statistically significant difference when compared with control. BMP-4: bone morphogenetic protein-4; SCAP: stem cells from apical papilla; MTT: 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide; CTGF: connective tissue growth factor; SPARC: secreted protein acidic and rich in cysteine.

Article Snippet: Effect of BMP-4 on the mRNA expression of various differentiation markers as analyzed by realtime PCR. (E) Osterix expression, (F) N-cadherin expression, (G) CTGF expression, (H) SPARC expression in SCAPs.

Techniques: MTT Assay, Control, Solvent, Cell Culture, Expressing

Effect of BMP-4 on uPA, uPAR, and PAI-1 mRNA expression, protein expression and production in SCAPs. (A) Exposure of SCAPs to BMP-4 decreased cellular uPA mRNA expression, (B) BMP-4 stimulated uPAR mRNA expression of SCAPs, (C) BMP-4 induced PAI-1 mRNA expression, ∗denotes statistically significant difference when compared with control ( P < 0.05) as analyzed by real-time PCR. (D) BMP-4 also inhibited uPA protein expression, (E) BMP-4 stimulated uPAR protein expression in SCAPs, (F) BMP-4 stimulated PAI-1 protein expression in SCAPs. One representative Western blot picture was shown. (G) Effect of BMP-4 on uPA production of SCAPs, (H) Effect of BMP-4 on suPAR production of SCAPs, (I) Effect of BMP-4 on PAI-1 production of SCAPs as analyzed by enzyme-linked immunosorbant assay. Results were expressed as Mean ± SE (pg/ml). ∗indicates statistically significant difference when compared with control ( P < 0.05). BMP-4: bone morphogenetic protein-4; uPA: urokinase plasminogen activator; uPAR: urokinase plasminogen activator receptor; PAI-1: plasminogen activator inhibitor-1; SCAP: stem cells from apical papilla; suPAR: soluble urokinase plasminogen activator receptor.

Journal: Journal of Dental Sciences

Article Title: Bone morphogenetic protein-4 induced matrix turnover and osteogenic differentiation-related molecules of stem cells from apical papilla and its associated ALK/Smad signaling

doi: 10.1016/j.jds.2024.11.002

Figure Lengend Snippet: Effect of BMP-4 on uPA, uPAR, and PAI-1 mRNA expression, protein expression and production in SCAPs. (A) Exposure of SCAPs to BMP-4 decreased cellular uPA mRNA expression, (B) BMP-4 stimulated uPAR mRNA expression of SCAPs, (C) BMP-4 induced PAI-1 mRNA expression, ∗denotes statistically significant difference when compared with control ( P < 0.05) as analyzed by real-time PCR. (D) BMP-4 also inhibited uPA protein expression, (E) BMP-4 stimulated uPAR protein expression in SCAPs, (F) BMP-4 stimulated PAI-1 protein expression in SCAPs. One representative Western blot picture was shown. (G) Effect of BMP-4 on uPA production of SCAPs, (H) Effect of BMP-4 on suPAR production of SCAPs, (I) Effect of BMP-4 on PAI-1 production of SCAPs as analyzed by enzyme-linked immunosorbant assay. Results were expressed as Mean ± SE (pg/ml). ∗indicates statistically significant difference when compared with control ( P < 0.05). BMP-4: bone morphogenetic protein-4; uPA: urokinase plasminogen activator; uPAR: urokinase plasminogen activator receptor; PAI-1: plasminogen activator inhibitor-1; SCAP: stem cells from apical papilla; suPAR: soluble urokinase plasminogen activator receptor.

Article Snippet: Effect of BMP-4 on the mRNA expression of various differentiation markers as analyzed by realtime PCR. (E) Osterix expression, (F) N-cadherin expression, (G) CTGF expression, (H) SPARC expression in SCAPs.

Techniques: Expressing, Control, Real-time Polymerase Chain Reaction, Western Blot

Effect of LDN193189 and SB431542 on BMP-4-induced Osx, N-cadherin, CTGF and SPARC expression in SCAPs. (A) Effect of LDN193189 on BMP-4-induced Osx mRNA expression, (B) Effect of SB431542 on BMP-4-induced Osx mRNA expression, (C) Effect of LDN193189 on BMP-4-induced Osx protein expression, (D) Effect of SB431542 on BMP-4-induced Osx protein expression. (E) Effect of LDN193189 on BMP-4-induced N-cadherin mRNA expression, (F) Effect of SB431542 on BMP-4-induced N-cadherin mRNA expression, (G) Effect of LDN193189 on BMP-4-induced CTGF mRNA expression, (H) Effect of SB431542 on BMP-4-induced CTGF mRNA expression, (I) Effect of LDN193189 on BMP-4-induced CTGF protein expression, (J) Effect of SB431542 on BMP-4-induced CTGF protein expression, (K) Effect of LDN193189 on BMP-4-induced SPARC mRNA expression, (L) Effect of SB431542 on BMP-4-induced SPARC mRNA expression. For western blotting, one representative result was shown. For real-time PCR, mRNA expression results were expressed as fold of control. ∗denotes statistically significant difference when compared with control. #denotes statistically significant difference when compared with BMP-4-treated group. BMP-4: bone morphogenetic protein-4; osx: osterix; connective tissue growth factor; SPARC: secreted protein acidic and rich in cysteine; SCAP: stem cells from apical papilla.

Journal: Journal of Dental Sciences

Article Title: Bone morphogenetic protein-4 induced matrix turnover and osteogenic differentiation-related molecules of stem cells from apical papilla and its associated ALK/Smad signaling

doi: 10.1016/j.jds.2024.11.002

Figure Lengend Snippet: Effect of LDN193189 and SB431542 on BMP-4-induced Osx, N-cadherin, CTGF and SPARC expression in SCAPs. (A) Effect of LDN193189 on BMP-4-induced Osx mRNA expression, (B) Effect of SB431542 on BMP-4-induced Osx mRNA expression, (C) Effect of LDN193189 on BMP-4-induced Osx protein expression, (D) Effect of SB431542 on BMP-4-induced Osx protein expression. (E) Effect of LDN193189 on BMP-4-induced N-cadherin mRNA expression, (F) Effect of SB431542 on BMP-4-induced N-cadherin mRNA expression, (G) Effect of LDN193189 on BMP-4-induced CTGF mRNA expression, (H) Effect of SB431542 on BMP-4-induced CTGF mRNA expression, (I) Effect of LDN193189 on BMP-4-induced CTGF protein expression, (J) Effect of SB431542 on BMP-4-induced CTGF protein expression, (K) Effect of LDN193189 on BMP-4-induced SPARC mRNA expression, (L) Effect of SB431542 on BMP-4-induced SPARC mRNA expression. For western blotting, one representative result was shown. For real-time PCR, mRNA expression results were expressed as fold of control. ∗denotes statistically significant difference when compared with control. #denotes statistically significant difference when compared with BMP-4-treated group. BMP-4: bone morphogenetic protein-4; osx: osterix; connective tissue growth factor; SPARC: secreted protein acidic and rich in cysteine; SCAP: stem cells from apical papilla.

Article Snippet: Effect of BMP-4 on the mRNA expression of various differentiation markers as analyzed by realtime PCR. (E) Osterix expression, (F) N-cadherin expression, (G) CTGF expression, (H) SPARC expression in SCAPs.

Techniques: Expressing, Western Blot, Real-time Polymerase Chain Reaction, Control